13 Biohacking Supplements Used by Elite Performers

The global biohacking market has exploded to USD 20.94 billion—and it’s accelerating at a staggering 18.6% annual growth rate through 2030¹. This isn’t just about having an edge anymore. It’s about survival in an era where human potential keeps expanding.

The supplement choices of elite performers matter because they’re the ones pushing the boundaries of human optimization. They experiment, iterate, and perfect protocols that eventually reshape our understanding of peak performance.

This isn’t another theoretical guide to supplements. We’ve analyzed the actual protocols of elite athletes, longevity researchers and professional biohackers to bring you the supplements that deliver measurable results.

The Rise of Elite Biohackers

Biohacking has evolved from a fringe movement into a sophisticated fusion of biology and technology—where elite performers deliberately optimize their bodies and minds through scientific experimentation. Silicon Valley’s titans aren’t just observers—they’re active participants and powerful catalysts.

The numbers tell a compelling story: Larry Ellison has invested over $330 million in aging research, while Larry Page launched Calico to push the boundaries of human lifespan. Peter Thiel has poured millions into life-extension therapies. These aren’t just investments—they’re declarations of intent.

The movement has transcended its Silicon Valley origins. Elite athletes now embrace advanced biohacking techniques—from whole-body cryotherapy to red-light therapy—revolutionizing their approach to performance and recovery. What began as experimental practice has evolved into a mainstream optimization toolkit, reshaping our understanding of human potential.

13 Biohacking Supplements Used by Top Performers

These aren’t just the best biohacking supplements—they’re the concentrated result of decades of research, validated by elite performers who stake their careers on optimizing human potential.

NAD+ Precursors

NAD+ (Nicotinamide Adenine Dinucleotide) is a fundamental coenzyme that powers cellular energy production and DNA repair. The molecule acts as your cells’ master switch for energy and renewal—but levels decline dramatically with age. NAD+ precursors like NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) are compounds that your body converts into NAD+ to effectively restore youthful levels of this critical molecule.

Research Highlights

• NAD+ precursors like NR and NMN have shown promising results in raising NAD+ levels during aging²
• Supplementation with NAD+ precursors has been found to improve physical exercise performance in older adults³
• NAD+ precursors significantly extended lifespan and improved neurological symptoms in mouse models, suggesting applications for both mitochondrial disorders and age-related decline⁴
• NMN supplementation has been shown to rescue mitochondrial and energy metabolism functions in aging mice⁵

Dosing Strategy

• Start with 250mg daily of NMN or NR
• Gradually increase to 500-1000mg based on response
• Take first thing in morning on empty stomach
• Consider cycling: 5 days on, 2 days off
• Advanced users may split into two daily doses
• Test NAD levels, monitor energy levels and sleep quality to optimize

Best paired with resveratrol and quercetin for improved NAD+ activation and cellular uptake.

Recommended Product: Our Vitality ↑® NAD+ Booster is a clinically-validated formulation that combines NAD precursors NMN and nicotinamide with Creatine monohydrate and D-Ribose to boost NAD+ levels more effectively than traditional supplements.

Creatine

Creatine isn’t just another sports supplement—it’s the most extensively studied performance enhancer in history. This naturally occurring compound serves as your cellular energy reserve, rapidly replenishing ATP—your body’s energy currency. Think of it as a biological battery backup that kicks in during intense physical and mental demands.

Research Highlights

• Meta-analyses show consistent 5-15% improvements in maximum power output⁶
• A meta-analysis found that creatine supplementation may improve short-term memory and intelligence/reasoning⁷
• Oral supplementation combined with resistance training increases maximal weight lifted in young men⁸
• Studies show accelerated muscle recovery and reduced post-exercise inflammation⁹
• New evidence suggests potential neuroprotective properties and brain health benefits¹⁰

Dosing Strategy

• Loading Phase (Optional): 20g daily split into 4 doses for 5-7 days
• Maintenance: 5g daily, consistent timing
• Use micronized creatine monohydrate for optimal absorption
• Take with protein and carbohydrates for enhanced uptake
• Mix with warm water for better dissolution
• Monitor hydration—increase water intake by 500ml per 5g creatine

Best combined with beta-alanine for power output and taurine for improved cellular hydration.

Recommended Product: Our advanced Creatine+ATP formula harnesses the synergistic power of pure creatine monohydrate and disodium ATP to optimize cellular energy production.

Curcumin

Curcumin represents the active powerhouse within turmeric—nature’s most potent anti-inflammatory compound. It acts as a master regulator of inflammation pathways, but its true magic lies in its ability to cross the blood-brain barrier and protect neural tissue. Traditional formulations barely scratch the surface of its potential—modern delivery systems have significantly improved its bioavailability.

Research Highlights

• Studies demonstrate reduction in exercise-induced inflammation comparable to NSAIDs¹¹
• Research indicates 28% improvement in memory tests in older adults¹²
• Curcumin supplementation increases serum BDNF in adult men and women¹³
• Recent data shows potential for optimizing mitochondrial function and cellular energy production¹⁴

Dosing Strategy

• Standard dose: 500-1000mg of bioavailable curcumin daily
• Must include black pepper extract (piperine) or use advanced delivery systems
• Split into 2-3 doses with fatty meals
• Optimal absorption with phospholipid-bound formulations
• Consider cycling: 6 weeks on, 2 weeks off
• Monitor inflammatory markers through regular blood work

Best paired with omega-3 fatty acids and quercetin for enhanced anti-inflammatory effects and cellular protection.

Recommended Product: Our premium turmeric curcumin supplement is formulated with a potent blend of 95% curcuminoids from turmeric extract, complemented by Boswellia, ginger root, quercetin, and piperine.

Magnesium

Magnesium isn’t just another mineral—it’s a fundamental conductor of over 300 enzymatic reactions in your body. Modern living depletes it. High-performers burn through it. Yet most supplementation fails because form matters more than quantity. Each magnesium compound targets different biological systems—from neural pathways to muscle function.

Research Highlights

• Magnesium threonate significantly improved deep sleep score and REM sleep score compared to placebo¹⁵
• Magnesium (300 mg daily) significantly reduced stress in severely stressed subjects with low magnesium levels¹⁶
• Suppressed oxidative stress and prevented DNA damage in rats exposed to chronic stress¹⁷
• Anti-inflammatory effects on the vascular endothelium, pointing to protection against atherosclerosis and cardiovascular diseases¹⁸

Dosing Strategy

• Magnesium Threonate: 2000mg (144mg elemental) for cognitive benefits
• Magnesium Glycinate: 400-600mg for sleep and recovery
• Magnesium Malate: 400mg for energy and exercise performance
• Take threonate in morning, glycinate at night
• Avoid taking with high-calcium foods
• Start low, increase gradually to assess tolerance
• Consider topical magnesium chloride for muscle recovery

Best combined with vitamin B6 for absorption and taurine for improved cellular uptake.

Quercetin/Fisetin

Quercetin and Fisetin belong to a powerful class of compounds called flavonoids—nature’s cellular cleanup crew. These senolytic supplements target and eliminate aged, dysfunctional cells that accelerate aging. But their power extends beyond longevity—they’re metabolic optimizers that enhance mitochondrial function and cellular resilience.

Research Highlights

• Quercetin has been shown to act as a strong antioxidant and anti-inflammatory agent¹⁹
• Fisetin reduced senescence in a subset of cells in murine and human adipose tissue²⁰
• Fisetin and Quercetin help kill cancer cells by inducing a process called apoptosis²¹
• Recent studies suggest enhanced NAD+ production when combined with NMN²²

Dosing Strategy

• Quercetin: 500-1000mg daily
• Fisetin: 500mg, taken as a “pulse dose”
• Pulse Protocol: Take fisetin for 2 consecutive days every month
• Always take with fat-containing meal
• Consider liposomal forms for improved absorption
• Stack with senolytic companion supplements for enhanced effects
• Optional: Cycle quercetin 5 days on, 2 days off

Best combined with senolytic activators like piperlongumine and NAD+ precursors for longevity benefits.

Recommended Product: SenoAid™ combines quercetin and fisetin flavonoids with bromelain enzymes, green tea extract, and piperine to target senescent cells and support cellular renewal.

CoQ10 (Coenzyme Q10)

CoQ10 stands as your cells’ primary energy catalyst—think of it as the spark plug in your cellular engine. As a critical component in the electron transport chain, it transforms nutrients into ATP. But CoQ10 isn’t just about energy—it’s one of nature’s most powerful antioxidants, protecting your cellular machinery from oxidative damage.

Research Highlights

• Moderate effectiveness in treating hypertension by decreasing total peripheral resistance and potentially lowering vascular superoxide levels²³
• Supplemental CoQ10 may help maintain physical activity levels and potentially extend the human life cycle²⁴
• CoQ10 demonstrates antioxidant properties and alleviates symptoms of conditions associated with mitochondrial dysfunction²⁵
• Shows promise in counteracting the reprotoxic effects of endocrine-disrupting chemicals like BPA²⁶

Dosing Strategy

• Standard dose: 100-200mg daily
• Athletes and high performers: 300-400mg daily
• Use ubiquinol form for superior absorption (especially over age 40)
• Take with fatty meals for optimal absorption
• Split larger doses into two daily servings
• Consider higher doses (400-600mg) for athletic performance
• Monitor energy levels to optimize timing

Best paired with PQQ (Pyrroloquinoline quinone) for enhanced mitochondrial biogenesis and function.

Omega-3s

Omega-3s are fundamental building blocks of cellular membranes and neural tissue. EPA and DHA, the two important forms, serve as the body’s primary anti-inflammatory signaling molecules. They literally reshape your cellular architecture, optimizing everything from neuron firing to muscle recovery.

Research Highlights

• Omega-3 fatty acids have been linked to healthy aging throughout life²⁷
• Higher intake and status of EPA and DHA are associated with a lower risk of incident type-2 diabetes and cardiovascular disease mortality²⁸
• EPA and DHA may positively affect various aspects of cardiovascular function, including inflammation reduction, peripheral artery disease prevention, and anticoagulation²⁹

Dosing Strategy

• Total daily dose: 2-4g EPA/DHA combined
• Aim for 2:1 EPA:DHA ratio for performance
• Take 1-2g with morning meal
• Take 1-2g with evening meal
• Store in cool, dark place
• Check for rancidity monthly
• Consider krill oil for enhanced absorption
• Test omega-3 index every 4-6 months

Best combined with vitamin D3 and K2 for enhanced absorption and cellular uptake.

Berberine

Berberine is nature’s answer to metformin—a glucose-disposal agent that doubles as a longevity compound. It activates AMPK, your body’s metabolic master switch, triggering a cascade of beneficial adaptations. Beyond blood sugar control, it rewires your cellular metabolism for enhanced energy efficiency and reduced aging markers.

Research Highlights

• Berberine has demonstrated significant anti-diabetic effects³⁰
• Berberine can effectively reduce body weight and improve lipid metabolism³¹
• Berberine may have neuroprotective effects, benefiting individuals with neurodegenerative diseases³²
• Clinical trials have shown that berberine can lower cholesterol levels³³

Dosing Strategy

• Standard dose: 500mg three times daily
• Take 15-30 minutes before meals
• Start with single 500mg dose to assess tolerance
• Build up gradually over two weeks
• Consider cycling: 8 weeks on, 2 weeks off
• Monitor blood glucose and lipids regularly
• Avoid taking with other AMPK activators

Best paired with alpha-lipoic acid for enhanced glucose control and chromium for improved insulin sensitivity.

Alpha-GPC

Alpha-GPC represents the most potent form of choline supplementation—a direct precursor to acetylcholine, your brain’s power switch for focus and muscle activation. Unlike other choline sources, Alpha-GPC crosses the blood-brain barrier with remarkable efficiency. It’s not just about brain power—it amplifies the mind-muscle connection, making it a dual-threat for both cognitive and physical performance.

Research Highlights

• Short-term intake improves memory and cognitive function in healthy college-age students³⁴
• Alpha-GPC may boost anaerobic power output after exhaustive exercise in healthy adults³⁵
• Enhances cognitive performance, particularly in mental rotation and n-back tests³⁶
• Alpha-GPC can be used to increase motivation in healthy individuals³⁷

Dosing Strategy

• Standard dose: 300-600mg daily
• Performance dose: 300mg 30-60 minutes pre-workout
• Cognitive dose: 150-300mg morning and afternoon
• Cycle usage: 5 days on, 2 days off
• Start low (150mg) to assess response
• Don’t exceed 1200mg daily
• Consider timing away from fatty meals

Best combined with Lion’s Mane and Bacopa Monnieri for enhanced cognitive benefits and neuroprotection.

Lion’s Mane

Lion’s Mane isn’t just another mushroom—it’s a neural regenerator that triggers the production of Nerve Growth Factor (NGF) and Brain-Derived Neurotrophic Factor (BDNF). These compounds act as fertilizer for your brain, stimulating the growth of new neural connections and protecting existing ones. While most nootropics focus on short-term performance, Lion’s Mane works on structural brain optimization.

Research Highlights

• Associated with improved mood stabilization and anxiety reduction in human studies³⁸
• Research suggests that Lion’s Mane may have positive effects on sleep enhancement in humans³⁹
• Clinical studies have demonstrated efficacy in neuroprotection and cognitive improvement in human subjects⁴⁰
• Studies indicate that Lion’s Mane may contribute to heart health and blood sugar regulation in humans⁴¹

Dosing Strategy

• Standard dose: 1000-2000mg daily
• Use dual-extracted products (both water and alcohol extraction)
• Look for minimum 30% beta-glucan content
• Take on empty stomach for optimal absorption
• Consider cycling: 4 weeks on, 1 week off
• Higher doses (3000mg+) for intensive cognitive demands
• Monitor effects through cognitive testing

Best combined with Niacin (B3) for enhanced neurotrophin production and absorption.

Resveratrol

Resveratrol functions as a cellular stress mimetic—triggering your body’s survival pathways without actual hardship. It activates the SIRT1 “longevity gene” from the family of sirtuins, essentially fooling your cells into a state of hormetic adaptation. While found naturally in red wine, supplemental forms deliver concentrated doses that activate these pathways more potently.

Research Highlights

• Clinical trials demonstrate resveratrol reduces inflammatory markers and improves aspects of dysregulated metabolism⁴²
• Data shows resveratrol improves lipid profiles, blood pressure, insulin resistance, and systemic inflammation⁴³
• Research demonstrates resveratrol’s ability to modulate multiple cell signaling molecules⁴⁴
• Studies indicate resveratrol has antioxidant, anti-inflammatory, and immunomodulatory effects⁴⁵

Dosing Strategy

• Standard dose: 500-1000mg daily
• Take with healthy fats for optimal absorption
• Morning dosing preferred
• Store in cool, dark place
• Use micronized or liposomal forms
• Avoid taking close to exercise
• Consider cycling: 5 days on, 2 days off

Best paired with NMN or NR for enhanced sirtuin activation and longevity benefits.

Vitamin K2 MK-4

K2 MK-4 isn’t just another vitamin—it’s the body’s calcium traffic director. While most focus on calcium intake, K2 ensures it lands in bones and teeth instead of arteries and soft tissues. This shorter-chain form of K2 executes a crucial biological program: activating proteins that govern calcium placement throughout your body’s architecture.

Research Highlights

• Clinical trials demonstrate MK-4 enhances cell viability and protects valvular interstitial cells from calcification⁴⁶
• MK-4 has the potential to induce differentiation in leukemia cells or apoptosis of various types of cancer cells⁴⁷
• Clinical trials reveal enhanced testosterone production⁴⁸
• Data indicates significant improvements in dental health⁴⁹
• Recent studies show potential cognitive protective effects⁵⁰

Dosing Strategy

• Standard dose: 1500-2000 mcg daily
• Take with meals containing healthy fats
• Split into 2-3 doses throughout day
• Must combine with Vitamin D3 (2000-5000 IU)
• Consider higher doses (up to 4000 mcg) for specific conditions
• Test vitamin D levels every 3-6 months
• Monitor calcium levels annually

Best paired with vitamin D3 and magnesium for optimal calcium metabolism and bone health.

Taurine

Taurine stands as one of your body’s most abundant free amino acids—yet it’s vastly underrated in the optimization world. It acts as an osmolyte, regulating cellular hydration and electrical signaling across membranes. Think of it as your cells’ internal pressure regulator and electrical conductor, optimizing everything from neural firing to muscle contraction.

Research Highlights

• Studies show taurine supplementation increases health span and potentially life span⁵¹
• Clinical trials demonstrate taurine’s protective effects against cardiovascular diseases⁵²
• Trials reveal enhanced muscle force production⁵³
• Clinical findings demonstrate taurine’s ability to improve lipid profiles and glucose metabolism⁵⁴

Dosing Strategy

• Standard dose: 2-3g daily
• Performance dose: 1g pre-workout, 2g post-workout
• Maximum daily dose: 6g
• Take on empty stomach for optimal absorption
• Consider pre-sleep dosing for enhanced recovery
• Combine with electrolytes during training
• Can split into multiple doses throughout day

Best paired with magnesium and B6 for enhanced absorption and neural benefits.

Optimizing Your Biohacking Supplement Protocol

Supplementation without measurement is like navigating without a map. Elite performers don’t guess—they test, adjust, and optimize based on precise biological data.

The AgingSOS® Advanced Longevity Blood Panel measures 22 critical biomarkers that most standard tests miss—from mitochondrial function to cellular senescence. This comprehensive panel doesn’t just identify current issues—it reveals hidden risk factors and validates your optimization efforts.

Your biohacking journey deserves precision. Transform your supplement protocol from experimental to evidence-based with the Advanced Panel.

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